Parameter |
Method of Analysis |
Output |
References |
Viscosity |
Rheological analysis |
Plot of changes in viscosity |
|
Cellular structure |
Microscopy |
Changes in cell/surface integrity |
Mandalari et al. (2014b) |
Phytochemicals bioaccessibility |
GC, HPLC |
Effect of food matrix on the bioaccessibility of bioactives from pistachios during simulated digestion |
Mandalari et al. (2013) |
Phytochemicals bioaccessibility |
HPLC-DAD |
Effect of food matrix and composition on polyphenol |
Udomwasinakun et al (2024)
|
Starch digestion |
Starch analysis |
Changes in ration of glucose: starch, total starch Efect of particle size on starch degradation from Durum wheat |
Ballance et al. (2013)
IFR, unpublished data |
Lipid separation |
Total lipid release and fatty acid analysis
|
Effect of mastication and processing on lipid release from almond seeds |
Mandalari et al. (2014b) |
Matrix restructuring and lipid bioaccessibility |
Lipid analysis and microscopy |
Role of gastric digestion on phase separation in foods containing different forms of fat
|
Salt et al (2023)
|
Protein digestion |
1D and 2D SDS-PAGE, RP-HPLC, MALDI-ToF, immunoblotting |
Effect of food matrix on protein digestion from almond seeds |
Mandalari et al. (2014b) |
Protein digestion
|
SDS-PAGE, protein analysis
|
Effect of processing on digestibility of milk proteins
|
Mulet-Cabero et al (2019)
|
Protein bioaccessibility |
Protein analysis, Microscopy
|
Protein release from a fungal cell matrix (mycoprotein) |
Colosimo et al (2020)
|
Starch digestion |
Enzyme kinetics, microscopy, sugar analysis
|
Mycoprotein inhibits starch digestibility which explains reduced glycaemic response in humans
|
Colosimo et al (2020) |
Beta-glucan release
|
Sugar analysis, microscopy
|
Comparison of beta-glucan release from plant and fungal sources and effect of digestive enzymes
|
Colosimo et al (2021)
|
Peptide production
|
1D and 2D SDS-PAGE, RP-HPLC, MALDI-ToF, immunoblotting
|
Persistence of allergens from cow’s milk and peanut flour
|
IFR, unpublished data
|
Probiotic survival
|
Culturing on selective media
|
Effect of food matrix on probiotic survival in the upper GI tract
|
Lo Curto et al. (2011) and Pitino et al. (2010, 2012)
|
Prebiotic delivery |
Culturing on selective media, genetic analysis
|
Delivery of potential prebiotics in the distal GI tract
|
Mandalari et al. (2010)
|